SrasV1, Main, Exploration, bibRecord, 002383

Cyclosporin A inhibits the replication of diverse coronaviruses

Identifieur interne : 002383 ( Main/Exploration ); précédent : 002382; suivant : 002384

Cyclosporin A inhibits the replication of diverse coronaviruses

Auteurs : Adriaan H. De Wilde [Pays-Bas] ; Jessika C. Zevenhoven-Dobbe [Pays-Bas] ; Yvonne Van Der Meer [Pays-Bas] ; Volker Thiel [Suisse] ; Krishna Narayanan [États-Unis] ; Shinji Makino [États-Unis] ; Eric J. Snijder [Pays-Bas] ; Martijn J. Van Hemert [Pays-Bas]

Source :

Journal of general virology [ 0022-1317 ] ; 2011.

RBID : Pascal:11-0480427

Descripteurs français

Pascal (Inist)
- Ciclosporine, Réplication, Immunodépresseur.

English descriptors

KwdEn :
- Ciclosporin, Immunosuppressive agent, Replication.

Abstract

Low micromolar, non-cytotoxic concentrations of cyclosporin A (CsA) strongly affected the replication of severe acute respiratory syndrome coronavirus (SARS-CoV), human coronavirus 229E and mouse hepatitis virus in cell culture, as was evident from the strong inhibition of GFP reporter gene expression and a reduction of up to 4 logs in progeny titres. Upon high-multiplicity infection, CsA treatment rendered SARS-CoV RNA and protein synthesis almost undetectable, suggesting an early block in replication. siRNA-mediated knockdown of the expression of the prominent CsA targets cyclophilin A and B did not affect SARS-CoV replication, suggesting either that these specific cyclophilin family members are dispensable or that the reduced expression levels suffice to support replication.

Affiliations:

Links toward previous steps (curation, corpus...)

to stream PascalFrancis, to step Corpus: 000086
to stream PascalFrancis, to step Curation: 000902
to stream PascalFrancis, to step Checkpoint: 000105
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Le document en format XML

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<front><div type="abstract" xml:lang="en">Low micromolar, non-cytotoxic concentrations of cyclosporin A (CsA) strongly affected the replication of severe acute respiratory syndrome coronavirus (SARS-CoV), human coronavirus 229E and mouse hepatitis virus in cell culture, as was evident from the strong inhibition of GFP reporter gene expression and a reduction of up to 4 logs in progeny titres. Upon high-multiplicity infection, CsA treatment rendered SARS-CoV RNA and protein synthesis almost undetectable, suggesting an early block in replication. siRNA-mediated knockdown of the expression of the prominent CsA targets cyclophilin A and B did not affect SARS-CoV replication, suggesting either that these specific cyclophilin family members are dispensable or that the reduced expression levels suffice to support replication.</div>
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Cyclosporin A inhibits the replication of diverse coronaviruses

Cyclosporin A inhibits the replication of diverse coronaviruses

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri